By Waldo E. Cohn (auth.), Seymour Rothchild (eds.)
The 11th Symposium on Advances in Tracer technique ology used to be held in Boston on October 13-14, 1966. The sym posium, which was once subsidized by means of the hot England Nuclear company, venerated the 10th anniversary of the company. The Symposia on Advances in Tracer technique, that have been held on a regular basis due to the fact that 1957, compile learn employees in numerous disciplines who percentage an curiosity in radioactive tracers. The 4 volumes, this one representing the list of the 11th Symposium, supply a compact and on hand resource of information-much of which was once frequently scattered in the course of the clinical literature-of inter est to biochemists, pharmacologists, endocrinologists, and analysts for whom tracers became an imperative software. The editor is principally thankful to Dr. Konrad Bloch, Harvard collage, and Dr. Waldo E. Cohn, Oak Ridge nationwide Laboratory, for chairing the classes on the 11th Sym posium. The cooperation of the audio system is deeply favored. moreover, a unique bouquet to my secretary, Mrs. Edith Thompson, for her beneficiant suggestions in arranging many of the symposia and the e-book of the papers. S. R.
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Additional info for Advances in Tracer Methodology: Volume 4: A collection of papers presented at the Eleventh Annual Symposium on Tracer Methodology
23. , Transition State, Spec. Publ. Chern. Soc. (London) 16 (1962). 24. , A Final Report on Ionization Potentials of Molecules by a Photoionization Method. Contrib. No. 480 and 737, University of Hawaii, Honolulu, 1959. 25. Matsen, F. , J. Chern. Phys. 24:602 (1956). 26. , Trans. Faraday Soc. 58:2452 (1962). 27. , Australian J. Chern. 18:1009 (1965). 28. Calf, G. , and Garnett, J. , unpublished data. 29. , Nature 201:902 (1964). 30. , J. Am. Chern. Soc. 76:5162 (1954). 31. , Tetrahedron Letters 3351 (1964).
A. Sollich-Baumgartner 23 catalyst is cheaper and easier to prepare and the method is thus to be preferred. Chloroplatinic acid is also reasonably specific in catalyzing exchange in the a -position with both hydrogen and borohydride reduction, although the more reactive borohydride catalyst also gives some f3 and y deuteration. Platinum oxide catalyzes exchange in all positions in pyridine, although the rate is fastest for the a-hydrogens . Confirmation of the nmr orientation is shown by the low-voltage mass spectrometry deuterium distribution patterns, for example, with cobalt there is a distinct cut-off at the d2 peak corresponding to the two a -hydrogens.
Rtition column chromatography . In designing the criteria of purity of the isolated cytidine-2-C 14 , the method of preparation and isolation was taken into consideration. The likely radioactive contaminants are cytosine-2-C 14 and the corresponding 2' and 3' ribonucleotides, and uracil-2-C 14 and its corresponding ribonucleoside and nucleotides. Although DNA should not be degraded by alkali, the paper chromatographic systems selected could also detect any contaminating deoxycytidine-2-C 14 • The presence of significant amounts of any unlabeled purines or their derivatives would be revealed by ultraviolet spectrophotometry.