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By Wee Ho Lim and Michael L. Roderick

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Gliomatosis is variably positive for GFAP and S100. 6 Electron Microscopy The finding of abundant intermediate filaments by ultrastructural analysis is characteristic. 7 Molecular Pathology There are both similarities and differences between adult and pediatric infiltrative astrocytomas from a molecular standpoint. ·· EGFR amplification is found in a significant proportion of primary glioblastomas in adults, particularly the small cell variant; in contradistinction, EGFR amplification is uncommon in pediatric glioblastomas.

B) This pontine glioblastoma has a heterogeneous red and tan mottled appearance with areas of hemorrhage and destructive necrosis. 5. (a) Smear preparation of a diffuse astrocytoma containing moderately cellular fragments of tissue and cells with oval hyperchromatic nuclei and elongated fibrillar cytoplasmic processes. (b) This smear preparation of diffuse astrocytoma demonstrates the gemistocytic morphology, the tumor cells having plump cytoplasmic “bellies”, some with discernible short processes, and eccentric round to oval nuclei.

6d,e). 6f); these may be highlighted by trichrome or periodic acid Schiff’s (PAS) stains. – Mitotic rate is low, and necrosis and endothelial/vascular proliferation are usually absent. 6g,h). – Rare anaplastic and giant cell variants have been described as case reports. 7b–d). – Perivascular pseudorosettes are quite uncommon, and true rosettes are not encountered. 7a), sclerotic vessels, and focal nuclear pleomorphism have all been described. – Approximately 20% of subependymomas will harbor areas of classic or even anaplastic ependymoma; in these instances, the lesions should be graded according to the highest grade component.

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